comparative evaluation of pcr and standard serological methods in detection of brucellosis in cattle

brusellosis is one of the most dangerous infectious zoonotic diseases which at present affect most areas of iran directly or indirectly.the aim of the present study was evaluate the efficacy of pcr test in detection of brucellosis. suspected animal sera (n=20) to brusellosis were collected from clinics and subjected to rose bengal and wright agglutination tests and to pcr analysis. while 6 samples (30%) were positive with titer 1:80,titer of the rest ones were less than 1:80. to confirm, all samples were subjected to pcr which revealed 5 samples of 1:80 and 6 samples of less than 1:80 were in agreement with wright test. however, 8 samples of 1:80 and 1 sample of less than 1:80 were in disagreement with wright test. the results of this study revealed that considerable percent of submitted samples were positive for brucellosis and positivity of ones were confirmed with pcr test we have shown 55% agreement between sat(standard agglutrination test) and pcr results.